GRAPE BIOTECHNOLOGY RESEARCH PROGRAM

MID-FLORIDA RESEARCH & EDUCATION CENTER

THE UNIVERSITY OF FLORIDA/IFAS

RECENT SELECTED PUBLICATIONS:

Patents (granted):

Gray, D. J. and R. Scorza, Disease resistance in Vitis , Australian Patent No. 723058 (34038.97), 2001.

Gray, D. J., J. Subramanian, and R E. Litz, Regeneration system for grape and uses thereof. South African Patent No. 2000/0591, 2001.

Scorza, R. and D. J. Gray, Disease resistance in Vitis , US Patent No. 6,232,528 B1, 2001.

Gray, D. J. and R. Scorza, Disease resistance in Vitis , Chilean Patent, 2002.

Gray, D. J., J. Subramanian, and R E. Litz, Regeneration system for grape and uses thereof. US Patent No. 6,455,312, 2002.

Gray, D. J. and R. Scorza, Disease resistance in Vitis , Mexican Patent No: 9810813, 2003.

Gray, D. J. and R. Scorza, Disease resistance in Vitis , European Patent No. 1138767, 2006.

Scorza, R. and D. J. Gray, Disease resistance in Vitis , Argentine Patent No: AR007631B1, 2006.

Subramanian, J, and D. J. Gray, Pathogen resistant grape plants. US Patent No. 6,995,015, 2006.

Books Edited:

Trigiano, R. N. and Gray, D. J., Editors, Plant Tissue Culture Concepts and Laboratory Exercises, CRC Press, Boca Raton , 1996, 374 pp.

Trigiano, R. N. and Gray, D. J., Editors, Plant Tissue Culture Concepts and Laboratory Exercises 2 nd Edition, CRC Press, Boca Raton, 2000, 430 pp.

Trigiano, R. N. and Gray, D. J., Editors, La Coltura dei Tessuti Vegetali, Edagricole, Bologna, 2003, 382 pp.

Trigiano, R. N. and Gray, D. J., Editors, Plant Development and Biotechnology, CRC Press, Boca Raton , 2005, 357 pp.

Book Chapters & Symposia:

Gray, D. J., Grape, In : S. M. Jain, P. K. Gupta, R. J. Newton (Eds.) Somatic Embryogenesis in Woody Plants, v.2. Kluwer Acad. Pub., 1995. pp.191-217.

Gray, D. J., S. Jayasankar, Z. Li, J. Cordts, R. Scorza and C. Srinivasan, Transgenic grapevines, In: G. G. Khachatourians, A. McHughen, R. Scorza, W. K. Nip and Y. H. Hui (Eds.), Transgenic Plants, Chapter 27, Marcel Dekker, 2002, pp. 397-405.

Gray, D. J., S. Jayasankar and Z. Li, Vitaceae (Grape Vitis spp.), Chap. 22, In : R. E. Litz (Ed.), Biotechnology of Fruit and Nut Crops, Biotechnology in Agriculture Series, No. 29, CAB International Wallingford, U.K., 2005, pp. 672-706.

Refereed Publications:

Gray, D. J., L. C. Fisher and J. A. Mortensen, Comparison of methodologies for in ovulo embryo rescue of seedless grape cultivars, HortScience 22, 1987, 1334-1335.

Gray, D. J. and J. A. Mortensen, Initiation and maintenance of long term somatic embryogenesis from anthers and ovaries of Vitis longii 'Microsperma', Plant Cell Tiss. Org. Cult. 9, 1987, 73-80.

Scorza, R., J. M. Cordts, D. J. Gray, D. Gonzalves, R. L. Emershad and D. W. Ramming, Production of transgenic 'Thompson Seedless' grape ( Vitis vinifera L.) plants, J. Amer. Soc. Hort. Sci. 121, 1996, 616-619.

Jayasankar S., D. J. Gray and R. E. Litz, High frequency somatic embryogenesis and plant regeneration in grapevine ( Vitis vinifera L.) using liquid suspension culture, Plant Cell Rep. 18, 1999, 533-537.

Jayasankar, S., Z. Li and D. J. Gray, In vitro selection of Vitis vinifera Chardonnay with Elsinoe ampellina is accompanied by fungal resistance and enhanced secretion of chitinase, Planta 211, 2000, 200-208.

Li, Z., S. Jayasankar and D. J. Gray, Expression of a bifunctional green fluorescent protein (GFP) fusion marker under the control of three constitutive promoters and enhanced derivatives in transgenic grape ( Vitis vinifera ), Plant Sci. 160, 2001, 877-887.

Li, Z., S. Jayasankar and D. J. Gray, An improved enzyme-linked immunoabsorbent assay protocol for the detection of small lytic peptides in transgenic grapevines ( Vitis vinifera ), Plant Molec. Biol. Reptr. 19, 2001, 341-351.

Jayasankar S., D. J. Gray and M. VanAman, Direct seeding of grapevine somatic embryos and regeneration of plants, In Vitro Dev. Biol. Plant 37, 2001, 476-479.

Jayasankar, S., B. R. Bondada, Z. Li and D. J. Gray, Comparative anatomy and morphology of Vitis vinifera (Vitaceae) somatic embryos from solid and liquid culture-derived proembryogenic cell masses, Amer. J. Bot. 90, 2003, 973-979.

Gray, D. J., Plant growth and development - artificial seeds. Encyclopedia of Applied Plant Science, Elsevier Science Ltd., Oxford , 2003, 1305-1310.

Jayasankar, S., Z. Li and D. J. Gray, Constitutive expression of Vitis vinifera thaumatin-like protein after in vitro selection and its role in anthracnose resistance, Functional Plant Biol. 30, 2003, 1105-1115.

Li, Z. and D. J. Gray, Effect of five antimicrobial peptides on the growth of Agrobacterium tumefaciens , Eschericia coli and Xylella fastidiosa , Vitis 42, 2003, 95-97.

Li, Z., S. Jayasankar and D. J. Gray, Bi-directional duplex promoters with duplicated enhancers significantly increase transgene expression in grape and tobacco. Trans. Res. 13, 2004, 143-154.

Gray, D. J., Z. Li and J. Subramanian, Tissue-specific expression of lytic peptides in transgenic grapevines via use of a GFP/NPTII fusion marker, Invited publication, 2 nd Intern. Symp. Biotechnol. Tropical and Subtropical Species, ACTA Hort., 692, 2005, 125-130.

Li, Z. and D. J. Gray, Isolation by improved TAIL-PCR and characterization of a seed-specific 2S albumin gene and its promoter from grape ( Vitis vinifera L.). Genome 48, 2005, 312-320.

Li, Z.T., S. Dhekney, M. Dutt, M. Van Aman, J. Tattersall, K.T. Kelley and D.J. Gray, Optimizing Agrobacterium -mediated transformation of grapevine using a bifunctional green fluorescent protein fusion marker, In Vitro Cell. Dev. Biol. Plant. 42, 2006, 220-227.

Dhekney, S.A., Z.T. Li., M. Van Aman, M. Dutt, J. Tattersall, and D.J. Gray, Genetic transformation of embryogenic cultures and recovery of transgenic plants in Vitis vinifera , Vitis rotundifolia and Vitis hybrids, 2005 Int. Symp. Biotechnol. Temperate Fruit Crops & Tropical Species, ACTA Hort. 2005 (submitted).

Dutt, M., Z.T. Li, K. Kelley, S.A. Dhekney, M. Van Aman, J. Tattersall, and D.J. Gray, Transgenic rootstock protein transmission in grapevines, 2005 Int. Symp. Biotechnol. Temperate Fruit Crops & Tropical Species, ACTA Hort. 2005 (submitted).

Gray, D. J., Z.T. Li, M. Dutt, S. A. Dhekney, M. Van Aman, J. Tattersall and K. T. Kelley, Screening transgenic grapevines for Pierce's disease resistance. 2005 Int. Symp. Biotechnol. Temperate Fruit Crops & Tropical Species, ACTA Hort. 2005 (submitted).

Li, Z.T., S.A. Dhekney, M. Dutt, M. Van Aman, J. Tattersall, K. Kelley and D.J. Gray, Isolation and characterization of the 2S albumin gene and promoter from grapevine. 2005 Int. Symp. Biotechnol. Temperate Fruit Crops & Tropical Species, ACTA Hort. 2005 (submitted)

Abstracts:

Gray, D. J., Z. J. Li, M. Van Aman, N. Barnett, A. Raveendran and K. Kelley, Applications of somatic embryogenesis and transformation in grapevine improvement, Invited presentation, 2003 Congress on In Vitro Biology, In Vitro Cell. Dev. Biol. 39, 2003, 7A.

Dutt, M., M. Van Aman and D. J. Gray, Liquid culture for rapid in vitro propagation of Vitis, 2004 Congress on In Vitro Biology, In Vitro Cell. Dev. Biol. 40, 2004, 56A.

Dhekney, S.A., Z.T. Li, M. Dutt, M.Van Aman and D.J. Gray, Genetic transformation and transgenic plant recovery from species of grape, 2005 Soc. In Vitro Biol. Ann. Meeting, In Vitro, 41, 2005, 28A.

Dhekney, S.A., Z.T. Li., M. Van Aman, M. Dutt, J. Tattersall, K.T. Kelley, and D.J. Gray, Genetic transformation of embryogenic cultures and recovery of transgenic plants in Vitis vinifera , Vitis rotundifolia and Vitis hybrids, 2005 Int. Symp. Biotechnol. Temperate Fruit Crops & Tropical Species, Program and Abstract Book, 41, 2005.

Dutt, M., Z.T. Li, K. Kelley, S.A. Dhekney, M. Van Aman, J. Tattersall, and D.J. Gray, Transgenic rootstock protein transmission in grapevines, 2005 Int. Symp. Biotechnol. Temperate Fruit Crops & Tropical Species, Program and Abstract Book, 50, 2005.

Gray, D. J., Z.T. Li, D.L. Hopkins, M. Dutt, S.A. Dhekney, M. M. Van Aman, J. Tattersall and K. T. Kelley, Transgenic Grapevines Resistant to Pierce's Disease, 2005 Amer. Soc. Hort. Sci. Ann. Meeting, HortScience, 40, 2005, 1104A.

Gray, D. J., Z.T. Li, M. Dutt, S. A. Dhekney, M. Van Aman, J. Tattersall and K. T. Kelley, Screening transgenic grapevines for Pierce's disease resistance. 2005 Int. Symp. Biotechnol. Temperate Fruit Crops & Tropical Species, Program and Abstract Book, 65, 2005.

Li, Z.T., S.A. Dhekney, M. Dutt, M. Van Aman, J. Tattersall, K. Kelley and D.J. Gray, Isolation and characterization of the 2S albumin gene and promoter from grapevine. 2005 Int. Symp. Biotechnol. Temperate Fruit Crops & Tropical Species, Program and Abstract Book, 86, 2005.

Dhekney , S.A. , Z.T. Li., M. Van Aman, M. Dutt, J. Tattersall, K.T. Kelley, and D.J. Gray, Disease resistant transgenic grapevine constitutively expresses Vitis vinifera thaumatin-like protein. 2006 Soc. In Vitro Biol. Ann. Meeting, Abstract Supplement.

Gray, D. J., Z.T. Li, S. A. Dhekney, M. Dutt, M. Van Aman, J. Tattersall and K. T. Kelley, Screening disease resistant transgenic grapevines for field tests. 2006 Soc. In Vitro Biol. Ann. Meeting, In Vitro, 42, 2006, 20A.

Dutt, M., D. J. Gray, Z . T. Li, S. Dhekney and M. M. Van Aman. Micropropagation cultures for genetic transformation of grapevine. HortScience 41(4), 2006, 972 A .

Dutt, M., Z. T. Li, S. Dhekney and D. J. Gray. Characterization of a composite promoter from genomic sequences of grapevine. HortScience 41(4), 2006,1053 A .