Faculty Information

 
Dennis J. Gray

Professor of Developmental Biology

University of Florida, IFAS
Mid-Florida Research & Education Center
2725 Binion Road, Apopka, FL 32703-8504

Phone: (407) 410-6946
Facsimile: (407) 814-6186
E-mail: djg@ufl.edu

  • 9/71-6/74 Modesto Junior College
  • 9/74-6/76 California State College, B.A. Biology
  • 8/77-7/79 Auburn University , M.S. Mycology, minor Botany
  • 8/79-5/82 North Carolina State University , Ph.D. Botany, minor Plant Pathology

Fields of Specialization

Developmental Biologist specializing in the study of regeneration phenomena from cells of crop plants. The crop of primary interest is grape, with secondary interest devoted to seedless watermelon and melon. I am pursuing the following research objectives:

1) Development of technology to accelerate creation of improved cultivars by incorporating non-conventional approaches such as cell culture, embryo rescue and genetic transformation into contemporary germplasm conservation and improvement programs.

2) Understanding the underlying mechanisms that lead to induction of embryogenic plant cells by utilizing highly refined regeneration systems for structural and molecular analyses.

Career Publications:

Professor Gray has published over 70 articles in refereed scientific journals and 30+ in non-referred publications. He has co-edited four popular textbooks covering the topics of plant tissue culture and biotechnology. He has contributed over 30 chapters to various books. He is frequently invited to speak at national and international scientific meetings, which have been summarized in over 80 published abstracts.

Gray is the Editor in Chief of “Critical Reviews in Plant Sciences”, a refereed journal with the 10 th highest impact rating among plant-related journals. He also is an Editorial Board member of “Plant Science” and a frequent reviewer for a host of other refereed journals. As leader of the UF Grape Biotechnology Research Laboratory, he received the 2002 USDA Secretary's Honor Awards for outstanding research accomplishments in grape biotechnology, which is the highest honor given yearly by that agency.

 

Selected Publications:

Patents (Granted):

Gray, D. J. and G. W. Elmstrom, Novel process for the accelerated production of triploid seeds for seedless watermelon cultivars, US Patent No. 5,007,198, 1991.

Gray, D. J. and R. Scorza, Disease resistance in Vitis , Australian Patent No. 723058 (34038.97), 2001.

Gray, D. J., J. Subramanian, and R E. Litz, Regeneration system for grape and uses thereof. South African Patent No. 2000/0591, 2001.

Scorza, R. and D. J. Gray, Disease resistance in Vitis , US Patent No. 6,232,528 B1, 2001.

Gray, D. J. and R. Scorza, Disease resistance in Vitis , Chilean Patent, 2002.

Gray, D. J., J. Subramanian, and R E. Litz, Regeneration system for grape and uses thereof. US Patent No. 6,455,312, 2002.

Gray, D. J. and R. Scorza, Disease resistance in Vitis , Mexican Patent No: 9810813, 2003.

Gray, D. J. and R. Scorza, Disease resistance in Vitis , European Patent No. 1138767, 2006.

Gray, D. J., J. Subramanian, and R E. Litz, Regeneration system for grape and uses thereof. Australian Patent Ser. No. 2002301408, 2006.

Li, Z. and D. J. Gray, Bi-directional dual promoter complex with enhanced promoter activity for transgene expression in eukaryotes, US Patent 7,129,343, 2006.

Scorza, R. and D. J. Gray, Disease resistance in Vitis , US Patent No. 7,151,203 B2, 2006.

Scorza, R. and D. J. Gray, Disease resistance in Vitis , Argentine Patent No: AR007631B1, 2006.

Subramanian, J, and D. J. Gray, Pathogen resistant grape plants. US Patent No. 6,995,015, 2006.

 

Patents (Pending):

Subramanian, J., D. J. Gray and R. E. Litz, Methods to generate improved grape cultivars. US Patent Application Serial No. 60/085,711, 1998.

Li, Z. and D. J. Gray, Bi-directional dual promoter complex with enhanced promoter activity for transgene expression in eukaryotes. International Application PCT/US02/0418 , 2002.

Gray, D. J. and Z. T. Li, Multi-functional genetic markers and uses thereof. Provisional US Patent Ser. No. 60/465,015, 2003. (abandoned).

Li, Z. T. and D. J. Gray, Nucleotide sequences of 2S albumen gene and its promoter and uses thereof. US Patent Application Ser. No. 10/382,066, 2003.

Li, Z. T. and D. J. Gray, Nucleotide sequences of 2S albumen gene and its promoter and uses thereof. International Application PCT/US2004/006765, 2004.

Li, Z. T. and D. J. Gray, A cercropin-melittin hybrid peptide with a reduced N-terminal extension confers high resistance to Xylella fastidiosa in transgenic grapevine. US Provisional Patent Application Ser. No. 60/689,937, 6/13/05.

Dutt, M. and D. J. Gray. 2006. A method for genetic transformation of grapevine and uses thereof. Invention disclosure submitted to UF for US Patent Application, 2006.

Li, Z. T. and D. J. Gray, Method of designing and utilizing hybrid peptides having antimicrobial activity. US Provisional Patent Application Ser. No. 60/887,636 2/1/07.

 

Books (Edited):

Trigiano, R. N. and Gray, D. J., Editors, Plant Tissue Culture Concepts and Laboratory Exercises, CRC Press, Boca Raton , 1996, 374 pp.

Trigiano, R. N. and Gray, D. J., Editors, Plant Tissue Culture Concepts and Laboratory Exercises 2nd Edition, CRC Press, Boca Raton, 2000, 430 pp.

Trigiano, R. N. and Gray, D. J., Editors, La Coltura dei Tessuti Vegetali, Edagricole, Bologna, 2003, 382 pp.

Trigiano, R. N. and Gray, D. J., Editors, Plant Development and Biotechnology, CRC Press, Boca Raton , 2005, 357 pp.

 

Book Chapters & Symposia:

Gray, D. J. and H. V. Amerson, In vitro colonization and resistance of loblolly pine embryos infected with the fusiform rust fungus, In: R. Henke, K. W. Hughes, M. J. Constantin, and A. Hollaender, Eds., Tissue Culture in Forestry and Agriculture, Plenum Press, New York , 1985, pp. 257-270.

Gray, D. J. and B. V. Conger, Somatic embryo ontogeny in tissue cultures of orchardgrass, In: R. Henke, K. W. Hughes, M. J. Constantin, and A. Hollaender, Eds., Tissue Culture in Forestry and Agriculture, Plenum Press, New York, 1985, pp. 49-57.

Conger, B. V., R. N. Trigiano and D. J. Gray, Cell culture of the Poaceae (Gramineae), Proc. NATO Advanced Study Institute on "Plant Cell Biotechnology", Vol. H18, Springer-Verlag, Heidelberg , 1988, pp. 49-61.

Gray, D. J., Introduction to the symposium, In: Proc. Symp. Synthetic Seed Technology for the Mass Cloning of Crop Plants: Problems and Perspectives, 22 nd Int. Hort. Cong., Davis, Calif., HortScience 22, 1987, pp. 796-797.

Gray, D. J., Somatic cell culture and embryogenesis in the Poaceae, Chapter 3, In : M. J. Kasperbauer (Ed.), Biotechnology in Tall Fescue Improvement, CRC Press, Boca Raton, FL, 1990, pp. 25-57.

Gray, D. J. and A. Purohit, Quiescence and dormancy in somatic embryos, In : Y. P. S. Bajaj (Ed.) Biotechnology in Agriculture and Forestry, vol. 17, High-tech and Micropropagation, I. Springer-Verlag, Heidelberg, 1991, pp. 382-394.

Gray, D. J., Synthetic seed for clonal propagation of crop plants, Proc. 3 rd Int. Symp. Seeds, In: R. B. Taylorson (Ed.) Recent Advances in the Development and Germination of Seeds, NATO ASI Series, Series A: Life Sciences, Plenum, New York , 1990, pp. 29-45.

Gray D. J. and C. P. Meredith, Grape biotechnology, In : F. A. Hammerschlag and R. E. Litz (Eds.) Biotechnology in Perennial Fruit Crops, CAB International Wallingford, U.K., 1992. pp. 229-262.

Gray, D. J. and M. E. Compton, Applications of synthetic seed technology to grape germplasm storage, In : K. Redenbaugh (Ed.) SynSeeds: Applications of Synthetic Seeds to Crop Improvement. CRC Press, Boca Raton , 1992. pp. 367-379.

Gray, D. J., R. N. Trigiano and B. V. Conger, Liquid suspension culture production of orchardgrass somatic embryos and their potential for the breeding of improved varieties, In: K. Redenbaugh (Ed.) SynSeeds: Applications of Synthetic Seeds to Crop Improvement. CRC Press, Boca Raton , 1992. pp. 351-366.

Litz, R. E. and D. J. Gray, Organogenesis and somatic embryogenesis, In : F. A. Hammerschlag and R. E. Litz (Eds.) Biotechnology in Perennial Fruit Crops, CAB International, Wallingford, U.K., 1992. pp. 3-34.

Gray, D. J., M. E. Compton, R. C. Harrell and D. J. Cantliffe, Somatic embryogenesis and the technology of synthetic seed, In : Y. P. S. Bajaj (Ed.) Biotechnology in Forestry and Agriculture. Springer, 1995. pp.126-151.

Gray, D. J., Grape, In : S. M. Jain, P. K. Gupta, R. J. Newton (Eds.) Somatic Embryogenesis in Woody Plants, v.2. Kluwer Acad. Pub., 1995. pp.191-217.

Capponetti, J. D., D. J. Gray and R. N. Trigiano, History of plant tissue culture, Topic chapter, In : R. N. Trigiano, D. J. Gray (Eds.), Plant Tissue Culture Concepts and Laboratory Exercises, CRC Press, 1996, pp. 3-8.

Compton , M. E. and D. J. Gray, Shoot organogenesis from cotyledon explants of watermelon, Laboratory exercise, In : R. N. Trigiano, D. J. Gray (Eds.), Plant Tissue Culture Concepts and Laboratory Exercises, CRC Press, 1996, pp. 113-119.

Gray, D. J., Photographic methods for plant tissue culture, Topic chapter, In : R. N. Trigiano, D. J. Gray (Eds.), Plant Tissue Culture Concepts and Laboratory Exercises, CRC Press, 1996, pp. 41-46.

Gray, D. J., Non-zygotic embryogenesis, Topic chapter, In : R. N. Trigiano, D. J. Gray (Eds.), Plant Tissue Culture Concepts and Laboratory Exercises, CRC Press, 1996, pp. 133-147.

Gray, D. J., Somatic embryogenesis from seeds of melon, Laboratory exercise, In : R. N. Trigiano, D. J. Gray (Eds.), Plant Tissue Culture Concepts and Laboratory Exercises, CRC Press, 1996, pp. 161-166.

Gray, D. J., B. V. Conger and R. N. Trigiano, Somatic embryogenesis from leaves of orchardgrass, Laboratory exercise, In : R. N. Trigiano and D. J. Gray (Eds.), Plant Tissue Culture Concepts and Laboratory Exercises, CRC Press, 1996, pp. 149-160.

Gray, D. J., M. E. Compton, E. Hiebert, C. M. Lin and V. P. Gaba, Construction and use of a simple gene gun for particle bombardment, Laboratory exercise, In : R. N. Trigiano and D. J. Gray (Eds.), Plant Tissue Culture Concepts and Laboratory Exercises, CRC Press, 1996, pp. 283-290.

Gray, D. J. and R. N. Trigiano, Introduction to plant tissue culture, In : R. N. Trigiano and D. J. Gray (Eds.), Plant Tissue Culture Concepts and Laboratory Exercises, 2 nd Edition, CRC Press, Boca Raton, 2000, pp. 3-7.

Capponetti, J. D., D. J. Gray and R. N. Trigiano, History of plant tissue culture, Topic chapter, In : R. N. Trigiano, D. J. Gray (Eds.), Plant Tissue Culture Concepts and Laboratory Exercises, 2 nd Edition, CRC Press, Boca Raton, 2000, pp. 11-17.

Compton , M. E. and D. J. Gray, Shoot organogenesis from cotyledon explants of watermelon, Laboratory exercise, In : R. N. Trigiano, D. J. Gray (Eds.), Plant Tissue Culture Concepts and Laboratory Exercises, 2 nd Edition, CRC Press, Boca Raton , 2000, pp. 149-157.

Gray, D. J., Photographic methods for plant tissue culture, Topic chapter, In : R. N. Trigiano, D. J. Gray (Eds.), Plant Tissue Culture Concepts and Laboratory Exercises, 2 nd Edition, CRC Press, Boca Raton, 2000, pp. 55-60.

Gray, D. J., Non-zygotic embryogenesis, Topic chapter, In : R. N. Trigiano, D. J. Gray (Eds.), Plant Tissue Culture Concepts and Laboratory Exercises, 2 nd Edition, CRC Press, Boca Raton, 2000, pp. 175-189.

Gray, D. J., Somatic embryogenesis from seeds of melon, Laboratory exercise, In : R. N. Trigiano, D. J. Gray (Eds.), Plant Tissue Culture Concepts and Laboratory Exercises, 2 nd Edition, CRC Press, Boca Raton, 2000, pp. 205-211.

Gray, D. J., R. N. Trigiano and B. V. Conger, Embryogenic callus and suspension cultures from leaves of orchardgrass, Laboratory exercise, In : R. N. Trigiano and D. J. Gray (Eds.), Plant Tissue Culture Concepts and Laboratory Exercises, 2 nd Edition, CRC Press, Boca Raton, 2000, pp. 191-203.

Gray, D. J., M. E. Compton, E. Hiebert, C. M. Lin and V. P. Gaba, Construction and use of a simple gene gun for particle bombardment, Laboratory exercise, In : R. N. Trigiano and D. J. Gray (Eds.), Plant Tissue Culture Concepts and Laboratory Exercises, 2 nd Edition, CRC Press, Boca Raton, 2000, pp. 329-336.

Gray, D. J., S. Jayasankar, Z. Li, J. Cordts, R. Scorza and C. Srinivasan, Transgenic grapevines, In: G. G. Khachatourians, A. McHughen, R. Scorza, W. K. Nip and Y. H. Hui (Eds.), Transgenic Plants, Chapter 27, Marcel Dekker, 2002, pp. 397-405.

Caponetti, J. S., D. J. Gray and R. N. Trigiano, Storia della coltura di tessuti vegetali, In: R. N. Trigiano and D. J. Gray (Eds.), La Coltura dei Tessuti Vegetali, Edagricole, Bologna, 2003, pp. 9-14.

Compton, M. E. and D. J. Gray, Organogenesi di germogli da espianti cotiledonari di anguria, In: R. N. Trigiano and D. J. Gray (Eds.), La Coltura dei Tessuti Vegetali, Edagricole, Bologna, 2003, pp. 129-136.

Gray, D. J., Techniche di fotographica applicate alla coltura di celle e tessuti vegetali, In: R. N. Trigiano and D. J. Gray (Eds.), La Coltura dei Tessuti Vegetali, Edagricole, Bologna, 2003, pp. 47-50.

Gray, D. J., Embriogenesi nonzigotica, In: R. N. Trigiano and D. J. Gray (Eds.), La Coltura dei Tessuti Vegetali, Edagricole, Bologna , 2003, pp. 149-160.

Gray, D. J., Embriogenesi somatica da semi di melone, In: R. N. Trigiano and D. J. Gray (Eds.), La Coltura dei Tessuti Vegetali, Edagricole, Bologna, 2003, pp. 173-178.

Gray, D. J., M. E. Compton, E. Hiebert, C-.M. Lin and V. P. Gaba, Construzione ed uso di semplice fucile genico per il bombardamento con particelle, In: R. N. Trigiano and D. J. Gray (Eds.), La Coltura dei Tessuti Vegetali, Edagricole, Bologna , 2003, pp. 283-290.

Gray, D. J. and R. N. Trigiano, Introduzione alla coltura di tessuti vegetali, In: R. N. Trigiano and D. J. Gray (Eds.), La Coltura dei Tessuti Vegetali, Edagricole, Bologna, 2003, pp. 3-6.

Gray, D. J., R. N. Trigiano and B. V. Conger, Callo embriogenico e colture in sospensione da foglie di erba mazzolina ( Dactylis glomerata L.), In: R. N. Trigiano and D. J. Gray (Eds.), La Coltura dei Tessuti Vegetali, Edagricole, Bologna, 2003, pp. 161-172.

Compton, M. E., D. J. Gray and V. P. Gaba, Genetic transformation of watermelon, Chap. 31, In: I. S. Curtis (Ed.), Transgenic Crops of the World, Kluwer Academic Publishers, Dordrecht, 2004, pp. 425-433.

Jayasankar, S. and D. J. Gray, In vitro plant pathology, In: R. N. Trigiano, M. T Windham and A. S. Windham (Eds.), Plant Pathology: Concepts and Laboratory Exercises, CRC Press, Boca Raton , 2004, pp. 367-377.

Capponetti, J. D., D. J. Gray and R. N. Trigiano, History of plant tissue culture, Chap. 2, In : R. N. Trigiano, D. J. Gray (Eds.), Plant Development and Biotechnology, CRC Press, Boca Raton, 2005, pp. 9-15.

Gray, D. J., Photographic methods for plant cell and tissue culture, Chap. 5, In : R. N. Trigiano, D. J. Gray (Eds.), Plant Development and Biotechnology, CRC Press, Boca Raton , 2005, pp. 49-54.

Gray, D. J., Propagation from non-meristematic tissues - Non-zygotic embryogenesis, Chap. 14, In : R. N. Trigiano, D. J. Gray (Eds.), Plant Development and Biotechnology, CRC Press, Boca Raton, 2005, pp. 187-200.

Gray, D. J., A simple illumination system for visualizing green fluorescent protein, Chap. 22, In : R. N. Trigiano, D. J. Gray (Eds.), Plant Development and Biotechnology, CRC Press, Boca Raton, 2005, pp. 273-276.

Gray, D. J., M. E. Compton, E. Hiebert, C. M. Lin and V. P. Gaba, Construction and use of a simple gene gun for particle bombardment, Chap. 21, In : R. N. Trigiano and D. J. Gray (Eds.), Plant Development and Biotechnology, CRC Press, Boca Raton, 2005, pp. 265-272.

Gray, D. J. and R. N. Trigiano, Introduction, Chap. 1, In : R. N. Trigiano and D. J. Gray (Eds.), Plant Development and Biotechnology, CRC Press, Boca Raton , 2005, pp. 3-6.

Jayasankar, S. and D. J. Gray, In vitro plant pathology, Chap. 25, In : R. N. Trigiano, D. J. Gray (Eds.), Plant Development and Biotechnology, CRC Press, Boca Raton, 2005, pp. 293-299.

Li, Z. T. and D. J. Gray, Genetic engineering technologies, Chap. 19, In : R. N. Trigiano and D. J. Gray (Eds.), Plant Development and Biotechnology, CRC Press, Boca Raton, 2005, pp. 241-250.

Trigiano, R. N. and D. J. Gray, Plant morphology and anatomy, Chap. 7, In : R. N. Trigiano and D. J. Gray (Eds.), Plant Development and Biotechnology, CRC Press, Boca Raton, 2005, pp. 73-85.

Gray, D. J., S. Jayasankar and Z. Li, Vitaceae (Grape Vitis spp.), Chap. 22, In : R. E. Litz (Ed.), Biotechnology of Fruit and Nut Crops, Biotechnology in Agriculture Series, No. 29, CAB International Wallingford, U.K., 2005, pp. 672-706.

 

Refereed Publications:

Morgan-Jones, G. and D. J. Gray, Notes on hyphomycetes, XXVIII, Mycogone psilocybina, a new mycoparasitic species, Mycotaxon 8, 1979, 144-148.

Gray, D. J. and G. Morgan-Jones, Notes on hyphomycetes, XXXIV, Some mycoparasitic species, Mycotaxon 10, 1980, 375-404.

Morgan-Jones, G. and D. J. Gray, Notes on hyphomycetes, XXXVIII, Sepedonium ampullosporum and Sibirina lutea sp. nov., Mycotaxon 12, 1980, 241-248.

Gray, D. J. and G. Morgan-Jones, Host-parasite relationships of Agaricus brunnescens and a number of mycoparasitic hyphomycetes, Mycopathologia 75, 1981, 55-59.

Gray, D. J., H. V. Amerson and C. G. Van Dyke, An ultrastructural comparison of monokaryotic and dikaryotic haustoria formed by the fusiform rust fungus Cronartium quercuum f. sp. fusiforme , Can. J. Bot. 60, 1982, 2914-2922.

Conger, B. V., G. E. Hanning, D. J. Gray and J. K. McDaniel, Direct embryogenesis from mesophyll cells of orchardgrass, Science 221, 1983, 850-851.

Gray, D. J. and H. V. Amerson, In vitro resistance of embryos of Pinus taeda to Cronartium quercuum f. sp. fusiforme , Phytopathology 73, 1983, 1492-1499.

Gray, D. J., H. V. Amerson and C. G. Van Dyke, Ultrastructure of the infection and early colonization of Pinus taeda by Cronartium quercuum f. sp. fusiforme , Mycologia 75, 1983, 117-130.

Trigiano, R. N., C. G. Van Dyke, H. W. Spurr Jr. and D. J. Gray, Infection and colonization of tobacco callus by Peronospora tabacina , Phytopathology 74, 1983, 280-295.

Gray, D. J., B. V. Conger and G. E. Hanning, Somatic embryogenesis in suspension and suspension-derived callus cultures of Dactylis glomerata , Protoplasma 122, 1984, 196-202.

Gray, D. J. and B. V. Conger, Influence of dicamba and casein hydrolysate on somatic embryo number and culture quality in cell suspensions of Dactylis glomerata (Gramineae), Plant Cell Tiss. Org. Cult. 4, 1985, 123-133.

Gray, D. J. and B. V. Conger, Time-lapse light photomicrography and scanning electron microscopy of somatic embryo ontogeny from cultured leaves of Dactylis glomerata (Gramineae), Trans. Amer. Microsc. Soc. 104, 1985, 395-399.

Gray, D. J., Quiescence in monocotyledonous and dicotyledonous somatic embryos induced by dehydration, In : Proc. Symp. Synthetic Seed Technology for the Mass Cloning of Crop Plants: Problems and Perspectives, 22nd. Int. Hort. Cong., Davis , Calif. , HortScience 22, 1987, 810-814.

Gray, D. J., B. V. Conger and D. D. Songstad, Desiccated quiescent somatic embryos of orchardgrass for use as synthetic seeds, In Vitro Cell. Dev. Biol. 23, 1987, 29-33.

Gray, D. J., L. C. Fisher and J. A. Mortensen, Comparison of methodologies for in ovulo embryo rescue of seedless grape cultivars, HortScience 22, 1987, 1334-1335.

Gray, D. J. and J. A. Mortensen, Initiation and maintenance of long term somatic embryogenesis from anthers and ovaries of Vitis longii 'Microsperma', Plant Cell Tiss. Org. Cult. 9, 1987, 73-80.

Mortensen, J. A. and D. J. Gray, 'Orlando Seedless' grape, HortScience 22, 1987, 327-328.

Conger, B. V., D. J. Gray and R. N. Trigiano, In vitro culture of the Gramineae, ACEVIV 3, 1988, 19-28.

Conger, B. V., J. C. Hovanesian, R. N. Trigiano and D. J. Gray, Somatic embryo ontogeny in suspension cultures of orchardgrass, Crop Sci. 29, 1989, 448-452.

Durham, R. E., G. A. Moore, D. J. Gray and J. A. Mortensen, The use of leaf GPI and IDH isozymes to examine the origin of polyembryony in cultured ovules of seedless grape, Plant Cell Rep. 7, 1989, 669-672.

Gray, D. J., Effects of dehydration and exogenous growth regulators on dormancy, quiescence and germination of grape somatic embryos, In Vitro Cell. Dev. Biol. 25, 1989, 1173-1178.

Trigiano, R. N., D. J. Gray, B. V. Conger and J. K. McDaniel, Origin of direct embryos from cultured leaf segments of Dactylis glomerata , Bot. Gaz. 150, 1989, 72-77.

Gray, D. J., J. A. Mortensen, C. M. Benton, R. E. Durham and G. A. Moore, Ovule culture to obtain progeny from hybrid seedless grapes, J. Amer. Soc. Hort. Sci. 115(6), 1990, 1019-1024.

Gray, D. J. and A. Purohit, Somatic embryogenesis and development of synthetic seed technology, Critical Rev. Plant Sci. 10, 1991, 33-61.

Gray, D. J. and C. M. Benton, In vitro micropropagation and plant establishment of muscadine grape varieties, Plant Cell Tiss. Org. Cult. 27,1991,7-14.

Gray, D. J., Somatic embryogenesis and plant regeneration from immature zygotic embryos of muscadine grape ( Vitis rotundifolia ) cultivars, Amer. J. Bot. 79, 1992, 542-546.

Compton , M. E., D. J. Gray and D. D. Songstad, Induction of quiescence in maize somatic embryos by dehydration, In Vitro Cell. Dev. Biol. Plant 28P, 1992, 197-201.

Compton, M. E. and D. J. Gray, Somatic embryogenesis and plant regeneration from immature cotyledons of watermelon, Plant Cell Rep. 12, 1993, 61-65.

Compton, M. E. and D. J. Gray, Shoot organogenesis and plant regeneration from cotyledons of diploid, triploid and tetraploid watermelon, J. Amer. Soc. Hort. Sci. 118, 1993, 151-157.

Compton, M. E. and D. J. Gray, A simple protocol for micropropagating diploid and tetraploid watermelon using shoot-tip explants, Plant Cell Tiss. Org. Cult. 33, 1993, 211-217.

Gray, D. J. and J. J. Finer, Development and operation of five particle guns for introduction of DNA into plant cells. Plant Cell Tiss. Org. Cult. 33, 1993, 219.

Gray, D. J. and L. A. Hanger, Effect of ovule maturity on recovery of zygotic embryos and embryogenic cultures from muscadine grape, HortScience 28, 1993, 227.

Gray, D. J., D. W. McColley and M. E. Compton, High-frequency somatic embryogenesis and plant regeneration from quiescent seed cotyledons of Cucumis melo cultivars, J. Amer. Soc. Hort. Sci. 118, 1993, 425-432.

Compton, M. E. and D. J. Gray, Adventitious shoot organogenesis and plant regeneration from cotyledons of tetraploid watermelon, HortScience 29, 1994, 211-213.

Gray, D. J., E. Hiebert, C. M. Lin, M. E. Compton and D. W. McColley, Simplified construction and performance of a device for particle bombardment, Plant Cell Tiss. Org. Cult. 37, 1994, 179-184.

Gray, D. J., R. N. Trigiano and B. V. Conger, Classroom exercises in the study of orchardgrass somatic embryogenesis, Proc. Workshop Novel Laboratory Exercises in Plant Tissue Culture, HortTechnology 4, 1994, 322-325.

Webb, S. E., M. L. Kok-Yokomi, D. J. Gray and C. M. Benton, In vitro rearing of grapevine aphid (Homoptera: Aphididae) on micropropagated shoot cultures of bunch grape and muscadine plants, Ann. Entomol. Soc. Amer. 87, 1994, 879-885.

Litz, R. E. and D. J. Gray, Somatic embryogenesis for agricultural improvement, World J. Microbiol. Biotechnol. 11, 1995, 416-425.

Monsalud, M.- J., H. Matthews, R. E. Litz and D. J. Gray, Control of hyperhydricity of mango somatic embryos, Plant Cell Tiss. Org. Cult. 42, 1995, 195-206.

Compton, M. E. and D. J. Gray, Identification of tetraploid regenerants from cotyledons of diploid watermelon cultured in vitro, Euphytica 45, 1996, 165-172.

Compton, M. E. and D. J. Gray, Effects of sucrose and methylglyoxal Bis- (guanylhydrazone) on controlling grape embryogenesis, Vitis 35, 1996, 1-6.

Gaba, V., C. Elman, A. A. Watad and D. J. Gray, Ancymidol hastens in vitro bud development in melon, HortScience 31, 1223-1224. 1996.

Pliego-Alfaro, F., R. E. Litz, P. A. Moon and D. J. Gray, Effect of abscisic acid, osmolarity and temperature on in vitro development of recalcitrant mango ( Mangifera indica L.) nucellar embryos, Plant Cell Tiss. Org. Cult. 44, 1996, 53-61.

Scorza, R., J. M. Cordts, D. J. Gray, D. Gonzalves, R. L. Emershad and D. W. Ramming, Production of transgenic 'Thompson Seedless' grape ( Vitis vinifera L.) plants, J. Amer. Soc. Hort. Sci. 121, 1996, 616-619.

Gal-On, A., E. Meiria, C. Elmana, D. J. Gray and V. Gaba, A simple hand-held device for the efficient infection of plants with viral-encoding constructs by particle bombardment, J. Virol. Methods 64, 1997, 103-110.

Gray, D. J., Breeding (plant), Invited article on genetic engineering of plants for disease resistance, McGraw-Hill Yearbook of Science and Technology, 1997, pp. 60-62.

Compton, M. E., N. Barnett and D. J. Gray, Use of fluorescein diacetate (FDA) to determine ploidy of in vitro watermelon shoots, Plant Cell Tiss. Org. Cult 58, 1999, 199-203.

Gaba, V., E. Schlarman, C. Elman, O. Sagee, A. A. Watad and D. J. Gray,

In vitro studies on the anatomy and morphology of bud regeneration in melon cotyledons, In Vitro Cell. Dev. Biol. Plant 35, 1999, 1-7.

Jayasankar S., D. J. Gray and R. E. Litz, High frequency somatic embryogenesis and plant regeneration in grapevine ( Vitis vinifera L.) using liquid suspension culture, Plant Cell Rep. 18, 1999, 533-537.

Jayasankar, S., Z. Li and D. J. Gray, In vitro selection of Vitis vinifera Chardonnay with Elsinoe ampellina is accompanied by fungal resistance and enhanced secretion of chitinase, Planta 211, 2000, 200-208.

Li, Z., S. Jayasankar and D. J. Gray, Expression of a bifunctional green fluorescent protein (GFP) fusion marker under the control of three constitutive promoters and enhanced derivatives in transgenic grape ( Vitis vinifera ), Plant Sci. 160, 2001, 877-887.

Li, Z., S. Jayasankar and D. J. Gray, An improved enzyme-linked immunoabsorbent assay protocol for the detection of small lytic peptides in transgenic grapevines ( Vitis vinifera ), Plant Molec. Biol. Reptr. 19, 2001, 341-351. (Note that the cover photograph for volume 19 is taken from our work).

Jayasankar S., D. J. Gray and M. VanAman, Direct seeding of grapevine somatic embryos and regeneration of plants, In Vitro Dev. Biol. Plant 37, 2001, 476-479.

Curuk, S., C. Elman, E. Schlarman , O. Sagee, I. Shomer, S. Cetiner, D. J. Gray and V. Gaba, A novel pathway for rapid shoot regeneration from the proximal zone of the hypocotyl of melon ( Cucumis melo L.), In Vitro Dev. Biol. Plant 38, 2002, 260-267.

Jayasankar S., B. Bondada, Z. Li and D. J. Gray, A unique morphotype of grape somatic embryos exhibits accelerated plant development, Plant Cell Rep. 20, 2002, 907-911.

Jayasankar, S., B. R. Bondada, Z. Li and D. J. Gray, Comparative anatomy and morphology of Vitis vinifera (Vitaceae) somatic embryos from solid and liquid culture-derived proembryogenic cell masses, Amer. J. Bot. 90, 2003, 973-979.

Gray, D. J., Plant growth and development - artificial seeds. Encyclopedia of Applied Plant Science, Elsevier Science Ltd., Oxford , 2003, 1305-1310.

Jayasankar, S. and D. J. Gray, In vitro selection of disease resistance in plants – an alternative to genetic engineering, Review Article, AgBiotecNet 5, 2003, 1 - 5.

Jayasankar, S., Z. Li and D. J. Gray, Constitutive expression of Vitis vinifera thaumatin-like protein after in vitro selection and its role in anthracnose resistance, Functional Plant Biol. 30, 2003, 1105-1115.

Li, Z. and D. J. Gray, Effect of five antimicrobial peptides on the growth of Agrobacterium tumefaciens , Eschericia coli and Xylella fastidiosa , Vitis 42, 2003, 95-97.

Compton, M. E., D. J. Gray and V. P. Gaba, Use of tissue culture and biotechnology for the genetic improvement of watermelon, Review of Plant Tissue Culture and Biotechnology, Plant Cell Tiss. Org. Cult. 77, 2004, 231-243.

Li, Z., S. Jayasankar and D. J. Gray, Bi-directional duplex promoters with duplicated enhancers significantly increase transgene expression in grape and tobacco. Trans. Res. 13, 2004, 143-154.

Gray, D. J., Z. Li and J. Subramanian, Tissue-specific expression of lytic peptides in transgenic grapevines via use of a GFP/NPTII fusion marker, Invited publication, 2 nd Intern. Symp. Biotechnol. Tropical and Subtropical Species, ACTA Hort., 692, 2005, 125-130.

Li, Z. and D. J. Gray, Isolation by improved TAIL-PCR and characterization of a seed-specific 2S albumin gene and its promoter from grape ( Vitis vinifera L.). Genome 48, 2005, 312-320.

Jayasankar S., M. Van Aman, J. Cordts, Z. Li and D. J. Gray, Long term storage of suspension culture-derived grapevine somatic embryos and regeneration of plants, In Vitro Cell. Dev. Biol. Plant. 41, 2005, 752-756.

Li, Z.T., S. Dhekney, M. Dutt, M. Van Aman, J. Tattersall, K.T. Kelley and D.J. Gray, Optimizing Agrobacterium -mediated transformation of grapevine, In Vitro Cell. Dev. Biol. Plant. 42, 220-227, 2006.

Dhekney, S.A., Z.T. Li., M. Van Aman, M. Dutt, J. Tattersall, and D.J. Gray, Genetic transformation of embryogenic cultures and recovery of transgenic plants in Vitis vinifera , Vitis rotundifolia and Vitis hybrids, 2005 Int. Symp. Biotechnol. Temperate Fruit Crops & Tropical Species, ACTA Hort. 2007 (in press).

Dutt, M., Z.T. Li, K. Kelley, S.A. Dhekney, M. Van Aman, J. Tattersall, and D.J. Gray, Transgenic rootstock protein transmission in grapevines, 2005 Int. Symp. Biotechnol. Temperate Fruit Crops & Tropical Species, ACTA Hort. 2007 (in press).

Gray, D. J., Z.T. Li, M. Dutt, S. A. Dhekney, M. Van Aman, J. Tattersall and K. T. Kelley, Screening transgenic grapevines for Pierce's disease resistance. 2005 Int. Symp. Biotechnol. Temperate Fruit Crops & Tropical Species, ACTA Hort. 2007 (in press).

Li, Z.T., S.A. Dhekney, M. Dutt, M. Van Aman, J. Tattersall, K. Kelley and D.J. Gray, Isolation and characterization of the 2S albumin gene and promoter from grapevine. 2005 Int. Symp. Biotechnol. Temperate Fruit Crops & Tropical Species, ACTA Hort. 2007 (in press).

 

Non-Refereed Publications:

Morgan-Jones, G., D. J. Gray and N. D. Davis, Species of Amanita: poisonous mushrooms, Highlights of Agric. Res., Auburn Univ., Agric. Expt. Sta. Bull. 25, 1978,16.

Gray, D. J. and G. Morgan-Jones, Fungi of Alabama, VIII, Some higher species from the Auburn area, J. Ala. Acad. Sci. 50, 1979, 225-230.

Conger, B. V. and D. J. Gray, Development of in vitro embryogenic culture systems for Dactylis glomerata , In: F. J. Novak, L. Havel, and J. Dolezel, eds, Proc. Int. Symp. Plant Tissue and Cell Culture: Application to Crop Improvement, Czech. Acad. Sci. Olomouc, Czechoslovakia , 1985, pp. 89-95.

Frampton Jr., L. J., H. V. Amerson and D. J. Gray, Development of in vitro techniques for screening loblolly pine for fusiform rust resistance, Proc. Rust of Hard Pines Working Party Conf., S2.06-10 I.U.F.R.O., Southeastern Forest Exp. Stn., 1985, pp.125-140.

Gray, D. J. and B. V. Conger, Nonzygotic embryogenesis in tissue cultures of forage grasses, Proc. 40th Southern Pasture and Forage Crop Improvement Conf., USDA-ARS, 1985, pp. 18-23.

Gray, D. J. and L. C. Fisher, In vitro shoot propagation of grape species, hybrids and cultivars, Proc. Fla. State Hort. Soc. 98, 1985, 172-174.

Conger, B. V., R. N. Trigiano and D. J. Gray, Dactylis glomerata : A potential model system for in vitro mutagenesis and mass propagation in cereals and grasses, Proc. Int. Symp. on Nuclear Tech. and In-Vitro Culture For Plant Imp., Int. Atomic Energy Agency, Vienna, Austria, 1986, pp. 371-383.

Gray, D. J., Applications of plant tissue culture to Florida viticulture, Proc. Viticultural Sci. Symp., Florida A & M Univ., Tallahassee, Fla., 1986, pp. 77-84.

Conger, B. V., R. N. Trigiano and D. J. Gray, Cell culture of the Poaceae (Gramineae), In: M.S.S. Pais, ed., Proc. NATO Advanced Study Institute on "Plant Cell Biotechnology", Vol. H18, Springer-Verlag, 1988, pp. 49-61.

Mortensen, J. A. and D. J. Gray, Orlando Seedless, a bunch grape for Florida, Univ. Fla. Agric. Expt. Sta. Circ. S-335, 1986, pp. 1-4.

Gray, D. J., Considerations for start-up commercial grape tissue culture, Proc. 1st Greater Grapes Symp., Univ. Florida, Gainesville., 1987, pp. 57-61.

Gray, D. J., Embryo rescue for seedless grape cultivar development, Proc. Viticultural Sci. Symp., Florida A&M Univ., Tallahassee, Fla., 1987, pp. 121-125.

Gray, D. J., Applications of plant tissue culture to viticulture in the Southeast, Proc. 2nd Ann. Viticultural Short Course, Mississippi State, MS, 1987, pp. 27-29.

Gray, D. J. and C. M. Klein, In vitro shoot micropropagation and plant establishment of 'Orlando Seedless' grape and ' Tampa ' rootstock, Proc. Fla. State Hort. Soc. 100, 1988, 308-309.

Gray, D. J. and C. M. Klein, In vitro micropropagation and plant establishment of 'Blanc Du Bois' grape, Proc. Fla. State Hort. Soc. 102, 1989, 221-223.

Bates, R. P., C. A. Sims, D. J. Gray and J. A. Mortensen, The Florida grape industry: What's ahead in the '90s?, Proc. Fla. State Hort. Soc. 103, 1990, 302-306.

Gray, D. J. and C. M. Benton, Micropropagation and plant establishment of muscadine grape, Proc. Fla. State Hort. Soc. 103, 1990, 300-302.

Gray, D. J., Hi-tech grapes, part 1: Overview of grape biotechnology research at the University of Florida , Florida Grape Growers Assn. Newsletter, May, 1990, p. 3.

Gray, D. J., C. M. Klein and M. Malibiran, Tissue culture micropropagation of muscadines, Proc. 1989 Viticultural Sci. Symp. FAMU, 1990, pp. 132-135.

Gray, D. J. and C. M. Benton, Tissue culture micropropagation of 'Blanc Du Bois' grape, Proc. 13th Ann. Viticultural Sci. Symp., FAMU, 1990, pp. 62-65.

Mortensen, J. A., D. J. Gray, L. H. Stover and J. W. Harris, Elite grape selections and cultivars developed at CFREC, Leesburg between 1945 and 1990, Proc. 13th Ann. Vitic. Sci. Symp., FAMU, 1990, pp. 106-112.

Gray, D. J., Biotechnology of muscadine grape, Proc. 1991 Florida Grape Growers Conference, 1992, pp. 141-144.

Compton , M.E. and D.J. Gray, Sucrose, abscisic acid and methylglyoxal Bis- (guanylhydrazone) affect grape somatic embryogenesis, Proc. Fla. State Hort. Soc. 106, 1993, 124-128.

Compton , M.E. and D.J. Gray, Micropropagation as a means of rapidly propagating triploid and tetraploid watermelon, Proceedings Florida State Horticultural Soc. 105, 1993, 352-354.

Gray, D. J., Progress toward development of seedless muscadine grapes,

FloridAgriculture 52, 1993, 23-24.

Compton , M. E. and D. J. Gray, Micropropagation of 'Southern Home' hybrid grape, Proc. Fla. State Hort. Soc. 107, 1994, 308-310.

Compton , M. E. and D. J. Gray, Regeneration of tetraploid plants from cotyledons of diploid watermelon, Proc. Fla. State Hort. Soc.107,1994, 107-109.

Meyerson , M.E. , C.M. Benton and D.J. Gray, A comparison of shoot micropropagation among bunch and muscadine grape species and cultivars, Proc. Fla. State Hort. Soc. 107, 1994, 311-312.

Gray, D. J., J. H. Harris, T. E. Crocker and K. T. Kelley, Effect of pruning on quality of 'Alachua' muscadine grape, Proc. Fla. State Hort. Soc. 109, 1996, 247-248.

Gray, D. J., Research vineyard at the University of Florida, Grape Times, Fla. Grape Growers Assoc. Newsletter, 7, 2002, 1.

Gray, D. J., First muscadine harvest at the new Mid-Florida Research and Education Center, Grape Times, Fla. Grape Growers Assoc. Newsletter, 2, 2004, 4.

Gray, D. J., A new website to support and promote Florida viticulture, Grape Times, Fla. Grape Growers Assoc. Newsletter, 10, 2004, 3.

 

Abstracts:

Gray, D. J. and G. Morgan-Jones, Some mycoparasitic fungi, A.I.B.S., Mycol. Soc. Amer. Newsletter 29, 1978, 32.

Gray, D. L. and G. Morgan-Jones, Some mycoparasitic hyphomycetes, Proc. Ala. Acad. Sci. 49, 1978, 63.

Gray, D. J., H. V. Amerson and C. G. Van Dyke, An ultrastructural comparison of monokaryotic and dikaryotic haustoria of the heteroecious southern fusiform rust ( Cronartium quercuum f. sp. fusiforme ), J. Elisha Mitchell Sci. Soc. 97, 1981, 248-249.

Gray, D. J., H. V. Amerson and C. G. Van Dyke, Ultrastructure of the infection and colonization of Pinus taeda by Cronartium quercuum f. sp. fusiforme (fusiform rust), A.I.B.S., Mycol. Soc. Amer. Newsletter 32, 1981, 33.





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